A NEW KYMOGRAM-BASED METHOD REVEALS UNEXPECTED EFFECTS OF MARKER PROTEIN EXPRESSION AND SPATIAL ANISOTROPY OF CYTOSKELETAL DYNAMICS IN PLANT CELL CORTEX

A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex

Abstract Background Cytoskeleton can be observed in live plant cells in situ with high spatial and temporal resolution using a combination of specific fluorescent protein tag expression and advanced microscopy methods such as spinning disc confocal microscopy (SDCM) or variable angle epifluorescence microscopy (VAEM).Existing methods liftmaster 850

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Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories

Copy number variation through gene or chromosome amplification provides a route for rapid phenotypic variation and supports the long-term evolution of gene functions.Although the evolutionary importance of copy-number variation is known, little is understood about how genetic background influences makita rf1101 its tolerance.Here, we measured fitne

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Lack of direct involvement of a diazepam long-term treatment in the occurrence of irreversible cognitive impairment: a pre-clinical approach

Abstract Several observational studies have found a link between the long-term use makita rf1101 of benzodiazepines and dementia, which remains controversial.Our study was designed to assess (i) whether the long-term use of benzodiazepines, at two different doses, has an irreversible effect on cognition, (ii) and whether there is an age-dependent e

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